MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3, -4 or HER-1, -2, -3, -4. It binds to epidermal growth factor (EGF), transforming growth factor-a (TGF-a), heparin binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is over expressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary and endometrium. It is predominantly present in squamous cell carcinomas.
Applications
Suitable for use in FLISA, Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.5-1ug/ml for 2 hrs at RT. Suitable for Western blot of extracellular domain of EGFR. Immunoprecipitation (Native only): 2ug/mg protein lysate. Use Protein G. Immunohistochemistry (Formalin/ Paraffin): 2-4ug/ml for 30 mniutes at RT. Staining of formalin-fixed tissues requires digestion with Protease XXV at 1mg/ml PBS for 5 min at 37°C. (Note: E3375-06B/ E3375-06BX are more suitable for use in IHC). Optimal dilutions to be determined by the researcher.
Positive Control
A431 cells, placenta or squamous cell carcinoma.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
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