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G1017-60 GAGA-POZ Domain, Recombinant, Drosophila melanogaster, aa1-130 CAS:

Specifications
References
Swiss Prot
Q08605
Grade
Highly Purified
Accession Number
NP_996080
Molecular Weight
14
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
Trithorax-like isoform A, Trl, Adf-2, GAGA, Nc70F, TFGAGA, GAGA-POZ domain, Trithorax-like protein, GAGA factor, GAF, Adh transcription factor 2, Neural conserved at 70F, Transcription factor GAGA, Trithorax-like isoform A, Trithorax like protein

The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo.

Recombinant protein corresponding to aa1-130 from drosophila melanogaster GAGA-POZ, expressed in E. coli. Swiss/Uniprot: Q08605
Molecular Weight
~14kD (130 amino acids)
Amino Acid Sequence
MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥ 95% (SDS PAGE); Purified by conventional column chromatography techniques.
Concentration
~1mg/ml (determined by Bradford assay)
Form
Supplied as a liquid in 10mM HEPES, pH 7.4, 25mM sodium chloride.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Kosoy, A., et al., J. Biol. Chem. 277(44): 42,280-42,288 (2002). |2. Espinas, M.L., et al., J. Biol. Chem. 274(23): 16,461-16,469 (1999). |3. Farkas, G., et al., Nature 371: 806-808 (1994).
USBio References
No references available
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