HCN3 CAS

Grade

Highly Purified

EU Commodity Code

38220090

Shipping Temp

Blue Ice

Storage Temp

-20°C

Control Peptide for H1827-27 (antiserum) and H1827-27A (affinity purified antibody).

Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life. Heart beating is the most reliable and rhythmic biological phenomenon. Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential. The hyperpolarization-activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization. The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq). The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity. Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel. The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels. HCNs proteins (HCN1-4) are characterized by six transmembrane domains (S1-S6), including a including a positively charged voltage-sensing S4 segment and an ion-conducting pore between S5 and S6. In the C terminus the HCNs carry a cyclic nucleotide-binding domain (CNBD), a motif found in several cyclic nucleotide-binding proteins. The core region of HCNs channels (S1 to the C terminus of the CNBD) is highly conserved, whereas the cytoplasmic N and C-termini, vary considerably in their length and share only weak sequence homology. The functional HCN, like other members of the superfamily of voltage-gated cation channels, channels probably assemble into tetrameric complexes. HCN3 or HAC3 (rat 780aa, mouse 779aa, human 703aa) has been detected in brain but its expression was much less than other HCNs. Its expression was also detected in liver, lung, and kidney of mouse by some researchers but not by others.

Applications

Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.

Recommended Dilution

Antibody Blocking: 5-10ug per 1ul H1827-27 (antiserum) or per 1ug H1827-27A (affinity purified antibody). ELISA: 50-100ng/well. Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Purity

Highly purified

Concentration

~1mg/ml

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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H1827-27B HCN3 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 3) Control Peptide CAS:

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