Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000-1:200,000 Western Blot: 1:5000-1:100,000 Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
References
1. Farr & Nakane, J. Immunol. Methods 47: 129-144, 1981.
TDS
NCBI
UniProt
Tech Support
Customer Service
