Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG molecule
Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:2500 to 1:25,000 Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.