Applications
Suitable for use in FLISA (Fluorescent) and Immunofluorescence. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by ion exchange chromatography.
Concentration
~1mg/ml (UV 280)
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Conjugated to Fluorescein isothiocyanate (FITC). Free FITC removed by solid phase adsorption.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Dog IgG and anti-Dog Serum.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.