Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
FLISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher.
Avidity for rat IgG2b: 4.7 x 10e9 M-1.
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Sato, K., et al., Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat transplant. J. Immunol. 166: 4185-4194 (2001).
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