Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 500-1000ng/ml Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.
Recommended Pair
Capture: I1904-84P Detection: K0100-01A
Hybridoma
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
References
1. Delauney, T., et al., Rat (and mouse) monoclonal antibodies. III. ELISA measurement of Ig production in mouse hybridoma culture supernatants. J. Immunol. Methods. 131: 33-39 (1990). 2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.|J Immunol. 185:7527-36.
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