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K1850-ML650 Kip1 p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) (MaxLight 650) CAS:

Specifications
References
Brand
MaxLight™
Swiss Prot
P46527
Grade
Affinity Purified
Accession Number
NM_004064
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: detects Kip1 (~27kD) in a HeLa nuclear extract and a rabbit brain nuclear extract. Immunoprecipitation: immunoprecipitates Kip1 from a HeLa nuclear extract and a rabbit brain nuclear extract. Immunohistochemistry: shows positive immunostaining for Kip1 in formalin-fixed, paraffin embedded rat brain tissue. Optimal dilutions to be determined by the researcher.
Positive Antigen Control
HeLa nuclear extract cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
Polyak, K., et al., Genes and Development 8: 9–22, 1994. Peters, G., Nature 371: 204–205, 1994.
USBio References
No references available
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