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L1185-11D-ML550 Lambda Light Chain (MaxLight 550) CAS:

Specifications
References
Brand
MaxLight™
Swiss Prot
P0CG04
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Applications
Suitable for use in Flow Cytometry and FLISA. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 cells or 100ul washed whole blood. Optimal working dilutions to be determined by researcher.
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Recommended Negative Control
I1904-78R: IgG1 Negative Control
Recommended Secondary Antibody
I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Reynolds, W M, et al. 1992. A simple technique for the determination of kappa and lambda immunoglobulin light chain expression by B cells in whole blood. J. Imm. Meth. 151:123-129.
USBio References
No references available
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