MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Nitric oxide (NO) is a potent bio-regulatory molecule which plays important roles in the regulation of a variety of normal developmental and physiological processes. NO is generated by nitric oxide synthase (NOS) in a reaction which converts L-arginine and oxygen into citrulline and nitric oxide. Three distinct mammalian genes have been identified which encode NOS isoforms: neuronal (nNOS or bNOS), macrophage or inducible (iNOS) and endothelial (eNOS). The NOS isoforms can be subdivided into two general categories, constitutive or inducible, based on differences in their regulation and activities. The constitutive isoforms include eNOS and nNOS. Although the nomenclature of the NOS family members suggests restricted isoform expression patterns, the160kD neuronal-type NO synthase (nNOS) has been detected in a variety of cell types including neurons, epithelial cells, mesangial cells and skeletal muscle cells.
Applications
Suitable for use in Western Blot, FLISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Western Blot (Rat): 1ug/ml FLISA: 0.1-1ug/ml Immunoprecipitation (Rat): 7ug/each Immunohistochemistry (Formalin- fixed paraffin): 2ug/ml. Tests using human, mouse and rat brain tissue sections yielded positive and specific cytoplasmic staining in neurons and astrocytes. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
TDS
NCBI
UniProt
Tech Support
Customer Service
