Suitable for use in Immunoprecipitation/Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunoprecipitation/Western Blot: 5ug immunoprecipitated PLC b-1 from a bovine brain membrane preparation. The immunoprecipitation procedure was followed by an Western Blot using 5ug/ml of P4074-40 and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Suh, P.G., et al., J. Biol. Chem. 263: 14497–14504, 1988. 2. Rhee, S.G., et al., Proc. Natl. Acad. Sci. USA 88: 5453, 1991. 3. Meisenhelder, J., Cell 57: 1109–1122, 1989. 4. Ryu, S.H., J. Biol. Chem. 262: 12511–12518, 1987. 5. Liu, et al, J Biol. Chem. 271: 355, 1996. 6. Kind, P.C., et al., J. Neurosci. 17: 1471-1480, 1987
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