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P4074-40-Biotin Phospholipase C, beta 1 (PLCB1, KIAA0581, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, Phospholipase C-beta-1) (Biotin) CAS:

Specifications
References
Swiss Prot
P10894
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Applications
Suitable for use in Immunoprecipitation/Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunoprecipitation/Western Blot: 5ug immunoprecipitated PLC b-1 from a bovine brain membrane preparation. The immunoprecipitation procedure was followed by an Western Blot using 5ug/ml of P4074-40 and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Suh, P.G., et al., J. Biol. Chem. 263: 14497–14504, 1988. 2. Rhee, S.G., et al., Proc. Natl. Acad. Sci. USA 88: 5453, 1991. 3. Meisenhelder, J., Cell 57: 1109–1122, 1989. 4. Ryu, S.H., J. Biol. Chem. 262: 12511–12518, 1987. 5. Liu, et al, J Biol. Chem. 271: 355, 1996. 6. Kind, P.C., et al., J. Neurosci. 17: 1471-1480, 1987
USBio References
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