Phosphotyrosine CAS

Brand

MaxLight™

Grade

Affinity Purified

EU Commodity Code

30021010

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.

Applications

Suitable for use in FLISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions

FLISA: 1:2000 Western Blot: 1:2000 Immunohistochemistry: 1:500 Immunocytochemistry: 1:500 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher.

Storage and Stability

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

References

1. Druker, B.J., H.J. Mamon, and T.M. Roberts (1989) New Eng. J. Med. 321:1382-1391. 2. Frackelton, R.A., Jr., A.H. Ross, and H.N. Eisen (1983) Mol. Cell. Biol. 3:1343-1352. 3. Glenney, J.R., Jr., L. Zokas, and M.P. Kamps (1988) J. Immunol. Meth. 109:277-285. 4. Hanissian, S.H. and N. Sahyoun (1992) J. Neurosci. Res. 32(4):576-582. 5. Takagi, S., M. Daibata, T.J. Last, R.E. Humphreys, D.C. Parker, and T. Sairenji (1991) J. Exp. Med. 174(2):381-388. 6. Tremblay, L. and R. Beliveau (1994) Am. J. Physiol. 267(3 Pt 2):F415-F422. 7. Wang, J.Y.J. (1988) Anal. Biochem. 172:1-7.

USBio References

No references available

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P4078-01A-ML650 Phosphotyrosine (MaxLight 650) CAS:

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