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P4275-03-ML650 Plk1 (STPK13, Polo-like kinase 1, Serine-threonine kinase 13) (MaxLight 650) CAS:

Specifications
Brand
MaxLight™
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Polo-like kinases (Plks) are important regulators of cell cycle progression during M-phase. Named after the polo gene of Drosophila, Plks are involved in the assembly and dynamics of the mitotic spindle apparatus and in the activation and inactivation of CDK/cyclin complexes. In mammalian cells, Plk1 activity levels increase as cells approach M phase, with the peak of phosphoylation activity reached during mitosis. Known substrates include Cdc25C phosphatase, cyclin B, a cohesion subunit of the mitotic spindle, subunits of the anaphase promoting complex, mammalian kinesin-like protein 1 (MKLP-1) and other kinesin related motor proteins. These substrates demonstrate the multiple roles of Plk1 in promoting mitosis. Plk1 has a role in the regulation of tyrosine dephosphorylation of CDKs through activation of Cdc25C. These sites of phosphorylation are distinct from the site phosphorylated by Chk1 kinase, which inactivates Cdc25C. Activation of CDK1 leads to spindle formation and M phase entry. When vertebrate cells enter prophase, cyclin B1 translocates from the cytoplasm to the nucleus. Phosphorylation of cyclin B1 by Plk1 on a serine residue in the middle of the nuclear export signal sequence is essential for translocation. During M-phase exit, Plk1 appears to be an important up-regulator of the ubiquitindependent proteolytic degradation machinery that controls passage through mitosis. The mechanism for this is not clear, but Plk1 can directly phosphorylate three subunits of the anaphase-promoting complex (APC), provided that Plk1 has been preactivated by CDK1/cyclin B. Finally, through its role in phosphorylation of kinesin-like proteins, Plk1 may also be important for cytokinesis. Plk1 can transform rodent cells, and it is frequently overexpressed in tumors, making it a potential marker for diagnostic or prognostic purposes.
Applications
Suitable for use in FLISA, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilution
FLISA: 0.1-1ug/ml Immunoprecipitation: 5-10ug/test Immunofluorescence: 0.5-2ug/test Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
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