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P4355-01-ML650 POH1 (Rpn11) (MaxLight 650) CAS:

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Western Blot: 1-3ug/ml Immunoprecipitation (Mouse): 7ug/IP reaction Optimal dilutions to be determined by the researcher.
Positive Controls
HeLa and NIH3T3
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Hershko A, et al. Annu Rev Biochem 67:425-479, 1998. 2. Zwickl P, et al. Curr Opin Struct Biol 10:242-250, 2000. 3. Yao T, et al. Nature 419:403-407, 2002. 4. Amerik AY, et al. Biol Chem 381:981-992, 2000. 5. D’Andrea A, et al. Crit Rev Biochem MolBiol 33:337-352, 1998. 6. Sptaro V, et al. J Biol Che 272:30470-30475, 1997.
USBio References
No references available
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