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P9009-59-ML650 Prolactin Receptor (PRLR, PRL-R, PRL Receptor) (MaxLight 650) CAS:

Specifications
References
Brand
MaxLight™
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Prolactin (PRL) is a neuroendocrine hormone that is closely related to growth hormones and more distantly related to the peptide hormones of the interleukin family. The endocrine effects of PRL on human breast tissues include the regulation of growth and differentiation of ductal epithelium, proliferation and differentiation of lobular units, and initiation and maintenance of lactation. PRL and growth hormone receptors are members of the cytokine receptor superfamily. Both the PRLr and growth hormone receptors are single-chain transmembrane proteins composed of extracellular, transmembrane, and intracellular domains. Prolactin modulates immune system function by stimulating the proliferation and survival of cells through its receptor, present on T and B lymphocytes and macrophages. The function of PRL is mediated by a variety of signaling cascades as well as by the wide variety of PRLr isoforms observed in nature. A long form and several other isoforms are expressed in human tissues. The nucleotide sequence of the novel intermediate isoform of PRLr is identical to the long isoform, except for a 573-base pair deletion occurring at a consensus splice site, resulting in a frameshift and truncated intracytoplasmic domain. The long isoform of PRLr is N-glycosylated. As the extracellular domain of the intermediate isoform is identical to that of the long isoform, glycosylation patterns are assumed to be similar. Using immunohistochemical analysis, PRLr expression has been observed in 95% of both normal and malignant breast epithelium; no correlation between PRL expression and ER-PR status has been established.
Applications
Sutiable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): 5-10ug/ml Immunoprecipitation: 5-10ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Kline, J.B., et al., J. Biol. Chem. 274(50): 35,461-35,468 (1999). 2. Kline, J.B., et al., J. Biol. Chem. 276(27): 24,760-24,766 (2001). 3. Pellegrini, I., et al., Mol. Endocrinol. 6(7): 1023-1031 (1992). 4. Clevenger, C.V., et al., Am. J. Pathol. 146(3): 695-705 (1995). 5. Clevenger, C.V., et al., J. Endocrinol. 157(2): 187-197 (1998). 6. Dorato, A., et al., Endocrinology 131(4): 1734-1742 (1992). 7. Reynolds, C., et al., Endocrinology 138(12): 5555-5560 (1997).
USBio References
No references available
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