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S7971-ML650 STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063) (MaxLight 650) CAS:

Specifications
References
Brand
MaxLight™
Swiss Prot
P40763
Grade
Affinity Purified
Accession Number
NM_139276
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Cytokines and growth factors bind to specific cellular receptors that induce differential phosphorylation and activation of proteins called STATs (for Signal Transducers and Activators of Transcription). STAT3 is phosphorylated on a tyrosine residue after treatment of cells with Epidermal Growth Factor and Interleukin-6. It is not phosphorylated after treatment of cells with Interferon gamma. Phosphorylated STAT3 can either form homodimers with itself or heterodimers with STAT1 alpha. These complexes can bind to specific sequences in nuclear DNA and likely modulate gene transcription.
Applications
Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Gel Shift Assay. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5-2ug/ml detects STAT3 in RIPA lysates from EGF stimulated human A431 cells and previously from mouse WEHI and rat L6. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT3 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 10ug/ml shows positive immunostaining for STAT3 in A431 cells fixed with 95% ethanol, 5% acetic acid. Immunoprecipitation: 4ug immunoprecipitates STAT 3 from 500ug of EGF-stimulated A431 RIPA lysate. Gel Shift Assay: This antibody supershifts. Optimal dilutions to be determined by researcher.
Positive Antigen Control (Included)
A0001-55: EGF-stimulated A431 cell lysate.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Ernst, M., et al., J. Biol. Chem. 274: 9729-9737 (1999). 2. Li, W., et al., J. Biol. Chem. 274: 9686-9691 (1999). 3. Yamamura, Y., et al., Mol. Cell. Biol. 18: 1172-1180 (1998). 4. Zong, Z., et al., Science 264: 95-98 (1994). 5. Darnell, Jr. J.E., et al., Science 264: 1415-1420 (1994). 6. Akira, S., et al., Cell 77: 63-71 (1994). 7. Zhang, E., et al., Science 267: 1990-1994 (1995).
USBio References
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