Syndecan 1 CAS

Brand

MaxLight™

Grade

Affinity Purified

EU Commodity Code

30021010

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Notes

Preservative Free
BSA Free

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Syndecans 1-4 are heparan sulfate proteoglycans (HSPGs), which are cell-surface-associated proteins. Syndecans contain a small core protein with distinct functional domains to which are linked multiple glycosaminoglycan (GAG) chains, predominantly of the heparan sulfate variety. Syndecan synthesis is highly regulated and is dependent on both cell type and developmental state. Syndecans are involved in cell adhesion, tissue morphogenesis, and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds. These functions are attributed primarily to the glycan moieties. Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor.

Applications

Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions

FLISA (Immunogen): 0.1-1ug/ml Western Blot (Human): 1-3ug/ml; Confirmed with human T47D, SW-480 and PC-3 cell lysates. A single band at ~90kD representing the glycosylated form of Syndecan 1 is observed and occasionally a band of ~31kD representing the unglycosylated form of Syndecan 1 may be observed. Immunohistochemistry (FFPE): 10ug/ml; For best results heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining. Confirmed with human gastric cancer and breast cancer tissues, as well as with mouse and rat stomach, small bowel and skin tissues. Optimal dilutions to be determined by the researcher.

Storage and Stability

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

References

1. Elenius, K., et al., J. Cell Biol. 114: 585-595 (1991). 2. Bayer-Garner, et al., J. Cutan. Pathol. 28: 135-139 (2001). 3. Conejo, J.R., et al., Int. J. Cancer 88: 12-20 (2000). 4. Kim, C.W., et al., Mol. Biol. Cell 5: 797-805 (1994). 5. Derksen, P.W., et al., Blood 99: 1405-1410 (2002). 6. Dhodapkar, M.V., et al., Br. J. Haematol. 99: 368-371 (1997). 7. Liebersbach, B.F., et al., J. Biol. Chem. 269: 20,013-20,019 (1994). 8. Matsuda, K., et al., Cancer Res. 61: 5562-5569 (2001). 9. Mukunyadzi, P., et al., Mod. Pathol. 15: 45-49 (2002). 10. Subramanian, S.V., et al., J. Biol. Chem. 272: 14,713-14,720 (1997). 11. Yeaman, C. & Rapraeger, A.C., J. Cell Biol. 122: 941-950 (1993). 12. Mundhenke, C., et al., Am. J. Pathol. 160: 185-194 (2002). 13. Ridley, R.C., et al., Blood 81: 767-774 (1993). 14. Seidel, C., et al., Blood 96: 3139-3146 (2000). 15. Stanley, M.J., et al., J. Biol. Chem. 270: 5077-5083 (1995).

USBio References

No references available

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S9111-40A-ML650 Syndecan 1 (MaxLight 650) CAS:

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