CTLL-2 Lysate

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Dry Ice

Storage Temp

-70°C

CTLL-2 cell line was a subclone of cytotoxic T-cells derived from a C57bl/6 mouse. CTLL-2 cells require IL-2 for growth and are used to assay for its presence in conditioned media. CTLL-2 cells are used also for the detection of many factors in cytokine assay.

Quality Control

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Preparation Before Usage

Heat lysate to 95°C for 5 minutes and rapidly cool before use.

Storage and Stability

Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Concentration

~2mg/ml (determined by BCA assay)

Form

Supplied as a SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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551903 CTLL-2 Lysate

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