Recognizes human CD240DCE and erythrocytes of normal Rh types but does not recognize Rhnull erythrocytes. Reacts with 31kD and 35-52kD proteins which were designated CD240DCE at the 7th leukocyte typing workshop.
Applications
FC IP
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
BSA Free
Yes
Preservative Free
Yes
Notes
Preservative Free BSA Free
Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Isotype Control
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Mouro-Chanteloup, I. et al. (2002) Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein. Blood. 100 (3): 1038-47. 2. Mouro-Chanteloup, I. et al. (2003) Evidence that the red cell skeleton protein 4.2 interacts with the Rh membrane complex member CD47. Blood. 101 (1): 338-44.
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NCBI
UniProt
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