Tissue genomic DNAs are isolated from a wide variety of tissues. The genomic DNAs are isolated from tissues by proprietary modified guanidine thiocyanate techniques. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris Cl, pH 7.5). Contamination by RNA, polysaccharides and proteoglycans has been effectively eliminated.
Features
Genomic DNA isolated from a wide variety of hard to obtain tissues Decontamination of polysaccharide and proteoglycan Extensive quality control procedures ensure high quality genomic DNA High efficiency in PCR Documentation of tissues' clinical histories available
Donor Information
Human Tumor Cell Line
Quality Control
1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, A260/230 is >2.0 (detected in 10mM Tris-Cl, pH 7.5). 2. RNAse treatment to ensure there is no RNA contamination. 3. Genomic DNA was successfully digested by Hind III. 4. B-Actin expression was tested by PCR amplification for Genomic DNA. 5. GAPDH expression was tested by Real Time PCR for 96 well tumor genomic DNA plate.
Applications
Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction and Profiling study in gene expression. Other applictions not tested.
Storage and Stability
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in 10mM Tris, pH 8, 1mM EDTA.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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