Interferon Gamma (IFNg) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFNg in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
15.6-1000pg/ml
Kit Components
*025981A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use *025981B: Standard, 2x1 vial 025981C: Standard Diluent, 1x20ml *025981D: Detection Reagent A, 1x120ul *025981E: Detection Reagent B, 1x120ul 025981F: Assay Diluent A, 1x12ml 025981G: Assay Diluent B, 1x12ml 025981H: TMB Substrate, 1x9ml 025981K: Stop Solution, 1x6ml 025981L: Wash Buffer, 30X, 1x20ml
Precaution
The Stop Solution (025981K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *025981A, *025981B, *025981D and *025981E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.