Protein Delta Homolog 1, pG2, Fetal Antigen 1, FA 1
DLK1, Soluble (human) BioAssay™ ELISA Kit (Protein Delta Homolog 1, pG2, Fetal Antigen 1, FA 1) is a sandwich ELISA used for the in vitro quantitative determination of human DLK1 in serum and cell culture supernatant.
Expected Values
DLK1 levels range in human serum from 0.4 to >2.5ng/ml (healthy donors)
Assay Range
0.469-30ng/ml
Sensitivity (Limit of Detection)
336pg/ml
Specificity
This ELISA is specific for the measurement of natural and recombinant human DLK1. It does not cross-react with human DLL1, human DNER, human adiponectin, human progranulin, human ANGPTL3, human RBP4, human resistin, human vaspin, human GPX3, human Nampt, human IL- 33, human clusterin, human leptin, human CTRP5, mouse Nampt, rat Nampt.
Assay Principle
This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human DLK1 in biological fluids. A monoclonal antibody specific for DLK1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, DLK1 is recognized by the addition of a purified polyclonal antibody specific for DLK1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to DLK1 in the samples.
Kit Components
044738A: Microtiter Strips, 1x96wells 044738B: Wash Buffer 10X, 2x30ml 044738C: ELISA Buffer 10X, 2x30ml 044738D: Detection Antibody, 1x20ul 044738E: IgG (HRP) 100X, 1x150ul *044738F: Standard, 1x60ug 044738G: TMB Solution, 1x12ml 044738H: Stop Solution 1N Sulfuric Acid, 1x12ml
Storage and Stability
Store *044738F powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.