Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDA adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. It has been shown that Nampt expression is increased in different diseases, like diabetes, obesity, fetal growth retardation, sepsis, inflammatory bowel disease, Crohn's disease and rheumatoid arthritis. Furthermore, Nampt might be a marker of pre-eclampsia and of endothelial health.
The Nampt Dual BioAssay™ ELISA Kit is to be used for the in vitro quantitative determination of (Visfatin/PBEF) in mouse or rat in serum.
Detection Range
0.5-32ng/ml
Specificity
Specific for the measurement of natural and recombinant mouse and rat Nampt. It does not crossreact with human Nampt, human adiponectin, mouse adiponectin, mouse resistin, mouse vaspin, mouse RBP4, mouse GPX3, mouse progranulin, mouse IL-33, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, mouse ANGPTL6, mouse leptin, mouse TNF-α.
Kit Components
045061A: Microtiter Strips, 1x96wells 045061B: Wash Buffer, 10X, 2x30ml 045061C: ELISA Buffer, 10X, 2x30ml 045061D: Detection Antibody, 1x50ul 045061E: IgG (HRP) 100X, 1x150ul 045061F: Standard, 1x64ng 045061G: TMB Solution, 1x12ml 045061H: Stop Solution, 1x12ml
Storage and Stability
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.