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137279 HDAC8, Fluorogenic Assay Kit, BioAssay™ (Histone Deacetylase 8, HD8, CDA07, HDAC8, HDACL1)

Specifications
Brand
BioAssay™
Kit Type
Assay
Tests
100
Detection Method
Colorimetric/Fluorescent
Sample Matrix
Cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C

The Fluorogenic HDAC 8 Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) 8 activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC8 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC 8 activity level. First, the HDAC fluorometric substrate is incubated with a sample containing HDAC activity (e.g. HeLa nuclear extract or purified HDAC8 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Intended Use
Designed to measure histone deacetylase (HDAC) 8 activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes.
Test Principle
The Fluorogenic HDAC 8 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays.
Kit Components
HDAC8 recombinant human enzyme: 1x2ug (0.6mg/ml) Fluorogenic HDAC substrate (5mM): 1x50ul 2x HDAC Developer (contains Trichostatin A): 1x6ml Trichostatin A (200uM) 1x100ul HDAC assay buffer: 1x10ml Black, low binding NUNC black microtiter plate: 1 plate
Storage and Stability
Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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