PCR, Cloning BioGenomics™ Kit

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137321 PCR, Cloning BioGenomics™ Kit

Specifications

Brand

BioAssay™

Kit Type

Assay

Tests

100

Detection Method

Colorimetric/Fluorescent

EU Commodity Code

38220000

Shipping Temp

Blue Ice

Storage Temp

-20°C

The PCR Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps.

Test Principle

A primer set homologous to the gene of interest and the vector is used to amplify a PCR product. This purified PCR product is incubated with restriction enzyme-digested vector and Reagent A of the Quick PCR cloning kit. Following a brief incubation with Reagent B, the annealed vector is ready for transformation.

Kit Components

Reagent A Reagent B Buffer A BSA10X (0.1% w/v) distilled water

Storage and Stability

Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.