Designed for identification of PDE1B inhibitors using fluorescence polarization.

The PDE1B inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE1B enzyme, fluorescently labeled PDE1B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE1B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

PDE1B: 1x1ug FAM-Cyclic-3’, 5’-AMP: 20uM: 1x50ul PDE assay buffer: 1x12ml Binding Agent: 1x100ul Binding Agent Diluent: 1x10ml Black, low binding NUNC black microtiter plate: 1 plate

Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.