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143748 CCL19/MIP-3 beta BioAssay™ ELISA Kit (Human)

Specifications
References
Brand
BioAssay™
Gene ID
6363
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
15.6pg/ml-1000pg/ml
Sample Matrix
cell culture supernates, serum and plasma (heparin, EDTA)
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Human CCL19/MIP-3 beta in cell culture supernates, serum and plasma (heparin, EDTA).

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta).  Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
Assay Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
Q6IBD6
Entrez Gene ID
6363
Kit Components
96-well plate pre-coated with anti-CCL19/MIP-3 beta, Human Lyophilized recombinant CCL19/MIP-3 beta, Human standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. Rossi, D. L., Vicari, A. P., Franz-Bacon, K., McClanahan, T. K., Zlotnik, A. Identification through bioinformatics of two new macrophage proinflammatory human chemokines. J. Immun. 158: 1033-1036, 1997. Note: Erratum: J. Immun. 163: 1091 only, 1999|2. Yoshida, R., Imai, T., Hieshima, K., Kusuda, J., Baba, M., Kitaura, M., Nishimura, M., Kakizaki, M., Nomiyama, H., Yoshie, O. Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific ligand for EBI1, CCR7. J. Biol. Chem. 272: 13803-13809, 1997. |
USBio References
No references available
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