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143803 Cystatin C BioAssay™ ELISA Kit, Mouse

Specifications
References
Brand
BioAssay™
Gene ID
13010
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
312pg/ml-20,000pg/ml
Sample Matrix
cell culture supernates, serum, plasma (heparin, EDTA) and urine
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Mouse Cystatin C in cell culture supernates, serum, plasma (heparin, EDTA) and urine.

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.
Assay Range
312pg/ml-20,000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
P21460
Entrez Gene ID
13010
Kit Components
96-well plate pre-coated with anti-Cystatin C, Mouse Lyophilized recombinant Cystatin C, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
1. Grubb, A.; Lofberg, H. : Mouse gamma-trace, a basic microprotein: amino acid sequence and presence in the adenohypophysis. Proc. Nat. Acad. Sci. 79: 3024-3027, 1982.|2. Pirttila, T. J.; Manninen, A.; Jutila, L.; Nissinen, J.; Kalviainen, R.; Vapalahti, M.; Immonen, A.; Paljarvi, L.; Karkola, K.; Alafuzoff, I.; Mervaala, E.; Pitkanen, A. : Cystatin C expression is associated with granule cell dispersion in epilepsy. Ann. Neurol. 58: 211-223, 2005.|3. Abrahamson, M. : Mouse cysteine proteinase inhibitors: isolation, physiological importance, inhibitory mechanism, gene structure and relation to hereditary cerebral hemorrhage. Scand. J. Clin. Lab. Invest. 48 (suppl. 191): 21-31, 1988.|4. Pirttila, T. J.; Manninen, A.; Jutila, L.; Nissinen, J.; Kalviainen, R.; Vapalahti, M.; Immonen, A.; Paljarvi, L.; Karkola, K.; Alafuzoff, I.; Mervaala, E.; Pitkanen, A. : Cystatin C expression is associated with granule cell dispersion in epilepsy. Ann. Neurol. 58: 211-223, 2005.|
USBio References
No references available
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