Protein A is a surface protein (approximately 56kD in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification.
Source
This product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein A.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:20,000-1:200,000 Western Blot: 1:10,000-1:40,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by Protein A affinity chromatography.
Concentration
~1mg/ml after reconstitution
Form
Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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