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171862 FOXO Reporter BioAssay™ Kit (PI3K/AKT Signaling Pathway)

Specifications
References
Brand
BioAssay™
Kit Type
Transfection
Tests
500
Detection Method
Luminescent
Sample Matrix
Cultured cells
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C

The FOXO Reporter BioAssay™ kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter kit also contains several control vectors for transfection efficiency and background luminescence.

Components
Reporter (Component A), FOXO luciferase reporter vector + constitutively expressing Renilla luciferase vector, 1x500ul (60ng DNA/ul) Negative Control Reporter (Component B), Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector, 1x500ul (60ng DNA/ul) FOXO3 (Component C), Expression vector for FOXO3, 1x250ul (100ng DNA/ul) Negative Control Expression Vector (Component D), Empty expression vector without FOXO3, 1x250ul (100ng DNA/ul)
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
References
1. Essaghir, A., et al. (2009). J. Biol. Chem. 284(16):10334-10342 2. 2. Hennessy B.T., et al. (2005). Nat. Rev. Drug Discov. 4(12):988-1004.
USBio References
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