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171995 SIRT5 (Sirtuin5) Fluorogenic BioAssay™ Kit

Specifications
References
Brand
BioAssay™
Kit Type
Kinetic
Tests
96
Detection Method
Fluorescent
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C/-70°C

The Fluorogenic SIRT5 BioAssay™ Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements. In addition, the kit includes purified SIRT5 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Applications
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Components
SIRT5 human recombinant enzyme, 1x50ug Fluorogenic SIRT5 substrate (5mM), 1x50ul Nicotinamide Adenine Dinucleotide (NAD+) (50mM), 1x50ul Nicotinamide (10mM), 1x500ul 2x SIRT Developer (contains 2mM Nicotinamide), 1x6ml SIRT assay buffer, 1x10ml black, low binding NUNC black microtiter plate
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
References
1. A. Ito et al. (2001) EMBO J. 20 1331. 2. A. Ito et al. (2002) EMBO J. 21 6236. 3. N.A. Barlev et al. (2001) Mol. Cell 8 1243.
USBio References
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