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214686 CD40

Specifications
References
Specificity
Recognizes bovine CD40. Species Crossreactivity: sheep
Source Antibody
bovine
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD40 cell surface antigen is expressed by B lymphocytes and a subset of T lymphocytes. CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.

Applications
Suitable for use in Flow Cytometry and Functional Assays. Other applications have not tested.
Recommended Dilution
Flow Cytometry: 1:25-1:200. 10ul labels 1x10e6 cells in 100ul Functional Assays: Sodium azide removal is recommended Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
References
1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70interaction with different bovine antigen-presenting cells. Scand J Immunol. 61: 242-50. 2. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP subset in lymph dendritic cells from the upper aero-digestive tract. J. Leukoc. Biol. 76: 553-561. 3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells. J. Gen. Virol. 84: 1771-1780. 4. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro. Immunology 108: 55- 61. 5. Haas, K.M. et al. (2001) Enhancing effects of anti-CD40 treatment on the immune response of SCID-bovine mice to Trypanosoma congolense infection. J Leukoc Biol. 70: 931-40.
USBio References
No references available
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