The PRMT3 Direct Activity Chemiluminescent BioAssay™ kit is designed to measure PRMT3 activity for screening and profiling applications.

The key to the PRMT3 Direct Activity BioAssay™ Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

DMSO >1%, strong acids or bases, ionic detergents, high salt

Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.

Plate precoated with histone substrate, 1x96 wells PRMT3 human recombinant enzyme, 1x10ug S-adenosylmethionine*, 20um, 1x250ul Primary antibody 4, 1x100ul Secondary HRP-labeled antibody 2, 1x10ul HMT assay buffer 2, 4x, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent A, (transparent bottle), 1x6ml HRP chemiluminescent B, (brown bottle), 1x6ml *Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.