The SUV39H2 Direct Activity BioAssay™ Kit is designed to measure SUV39H2 activity for profiling and screening applications, using purified SUV39H2.

The SUV39H2 Direct Activity BioAssay™ Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H2 enzyme for 100 enzyme reactions. The key to the SUV39H2 Activity BioAssay™ Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate is added to produce chemiluminescence that can then be measured using a chemiluminescence reader.

DMSO >1%, strong acids or bases, ionic detergents, high salt

Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.

Plate precoated with histone substrate, 1x96 wells SUV39H2, 1x4ug 100uM S-adenosylmethionine 1x250ul Primary antibody 1, 1x100ul Secondary HRP-labeled antibody 1, 1x10ul 4x HMT Assay Buffer 1, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate, 2 components x 6ml each

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.