DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling.
Intended Use
Direct Assays: plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices.
Range
2-40ng (100-2000ng/ml) calf thymus DNA
Test Principle
This DNA BioAssay™ kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA follow ing polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double stranded DNA. The fluorescence intensity, measured at 450nm (lexc = 350nm), is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Kit Components
Reagent, 1x50ml Standard, 10mg/ml, calf thymus DNA, 1x1ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TDS
NCBI
UniProt
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