Formalin-fixed, paraffin embedded (FFPE) tissue specimens are highly valuable sources for retrospective studies of many pathologies. Nevertheless, the extraction of nucleic acids from FFPE specimens could often be challenging, as nucleic acids become cross-linked and degraded during the archiving process. Nucleic acids obtained are usually highly fragmented and chemically modified from the archiving process.
Test Principle
This FFPE Tissue DNA Extraction BioAssay™ Kit - Column allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues, with potential high throughput capabilities and full compatibility for downstream applications such as qPCR. Utilizing heat and proteinase K treatment, The FFPE Tissue DNA Extraction Kit- Column is optimized in the removal of paraffin, partial reversal of formalin cross linking, and release of DNA from fixed tissues. After clean up with DNA binding columns, the concentration of the high purity DNA can be determined by spectrophotometer or nanodrop equipment.
Kit Components
Dewaxil, 1x28ml Proteinase K, 1x1.1ml FFPE Lysis Buffer, 1x10ml Binding Buffer, 1x11ml Wash Buffer 1, 1x bottle Wash Buffer 2, 1x bottle Elution Buffer, 1x2.8ml Column, 50x columns Actin control primer,1x tube
Quality Control
All kit components are DNase-, RNase-, and protease-free. Each component has been tested for purity and efficacy.
Storage and Stability
Aliquot Proteinase K into appropriate amounts and store aliquots and actin control primer at -20°C upon receipt of the kit. Store all other components at room temperature.
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