Path-B.mallei-EASY
Product features
• Exceptional value for money • Rapid detection of all clinically relevant subtypes • Positive copy no. standard curve for quantification • Highly specific detection profile • High priming efficiency • Broad dynamic detection range (>6 logs) • Sensitive to < 100 copies of target • Accurate controls to confirm findings
PRODUCT MANUAL
http://www.genesig.com/assets/files/b_mallei_easy.pdf
EASY KIT COMPONENTS
• B mallei specific primer/probe mix (BROWN) Once resuspended the kits should remain at --20°C until ready to use. • Lyophilised oasig Mastermix • Lyophilised oasig Mastermix resuspension buffer (BLUE lid) • B mallei . positive control template (RED lid) • Internal extraction control RNA (BLUE lid) • RNAse/DNAse free water (WHITE lid ) •54x genesig® q16 reaction tubes
REAGENTS AND EQUIPMENT TO BE SUPPLIED BY THE USER
• genesig® q16 instrument • genesig® Easy Extraction Kit This kit is designed to work well with all processes that yield high quality RNA but the genesig® Easy extraction method is recommended for ease of use. • genesig® Lab-In-A-Box The genesig® Lab-In-A-Box contains all of the pipettes, tips and racks that you will need to use a genesig® Easy kit. Alternatively if you already have these components and equipment these can be used instead.
INSTRUMENTATION REQUIREMENTS
Designed specifically for the genesig® q16, genesig®. Easy kits are ultra simple and easy to use for users of all experience levels.
ABOUT THE genesig® KITS
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative Real-Time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented Real-Time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above. Packaged, optimized and ready to use.
DNA/RNA EXTRACTION
genesig® Easy DNA/RNA extraction kits are a simple to use, low cost and powerful extraction technology suitable for virtually all sample types.