This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.
Sample Type
Serum, plasma, tissue homogenates
Intended Use
For the quantitative determination of human transforming growth factor-beta-induced protein ig-h3 (TGFBI/BIGH3) concentrations in serum, plasma and tissue homogenates.
Specificity
This assay has high sensitivity and excellent specificity for detection of human TGFBI. No significant cross-reactivity or interference between human TGFBI and analogues was observed.
Test Principle
This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGFBI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFBI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Kit Components
Assay plate (12x8 coated microwells), 1x96 wells Standard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x10ml HRP-avidin Diluent, 1x10ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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