SABC (StreptAvidin-Biotin Complex) is specially designed for displaying the distribution of antigens in tissues and cells in immunochemistry and other immunodetection analyses. This kit has high sensitivity because each complex it generates has a large number of FITC and streptavidin molecules. Compared to the traditional immunodetection using free FITC dyes, the SABC-FITC system greatly enhances the sensitivity and suppresses the background. FITC excitation is at 490~495nm, emission at 520~530nm.
Kit Components
1. Normal goat serum blocking reagent: 10x, 10ml, for the block of tissue sections. 2. Biotinylated Secondary Antibody (Goat Anti-mouse IgM): 100~200x, 1ml (2mg/ml). Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). 3. SABC-FITC (FITC conjugated streptavidin): 100~200x, 1ml (2mg/ml). 4. Three drop bottles (For dilution use).
Applications
Suitable for use in Immunohistochemistry (paraffin), Immunohistochemistry (frozen), Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1:50-200 Immunohistochemistry (frozen): 1:50-200 Immunocytochemistry: 1:50-200 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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