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352949 JAM-A/F11R, BioAssay™ ELISA Kit (Mouse) (Junctional Adhesion Molecule A)

Specifications
Brand
BioAssay™
Gene ID
16456
Kit Type
Sandwich ELISA
Tests
96
Sample Volume
50-100ul
Sensitivity
<10pg/ml
Detection Method
Colorimetric
Detection Range
31.2-2000pg/ml
Sample Matrix
Cell culture supernates, serum and plasma (heparin, EDTA)
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Intended Use
For quantitative detection of mouse JAM-A in cell culture supernates, serum and plasma (heparin, EDTA).
Sensitivity
<10pg/ml
Range
31.2-2000pg/ml
Specificity
Natural and recombinant mouse JAM-A
Test Principle
This mouse JAM-A BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for JAM-A has been precoated onto 96-well plates. Standards(NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for JAM-A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse JAM-A amount of sample captured in plate.
Kit Components
96-well plate precoated with mouse JAM-A antibody, 1 Lyophilized recombinant mouse JAM-A standard, 10ng/tube×2 Biotinylated mouse JAM-A antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Mouse
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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