246.9-20,000ng/ml

87.2ng/ml

<10%

<12%

*365783A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use. *365783B: Standard, 2x1vial 365783C: Standard Diluent, 1x20ml *365783D: Detection Reagent A (green), 1x120ul *365783E: Detection Reagent B (red), 1x120ul 365783F: Assay Diluent A, 1x12ml 365783G: Assay Diluent B, 1x12ml 365783H: TMB Substrate, 1x9ml *365783J: Positive Control, 1248ng/ml, 1x1vial (Lyophilized) 365783K: Stop Solution, 1x6ml 365783L: Wash Buffer, 30X, 1x20ml

The Stop Solution (365783K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Store *365783A, *365783B, *365783D, *365783E and *365783J at -20°C. After reconstitution of *365783J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 365783J: Positive Control

The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

The positive control vial contains human BSA in human serum (lyophilized powder). Note: The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

1248ng/ml

Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.

1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C. 7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.