0.69-500pg/ml

0.28pg/ml

This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF). No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed.

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the EGF level in the sample or standard.

<10%

<12%

Microtiter Plate: 1x96 wells Standard, 2x1 vial Standard Diluent, 1x20ml Detection Reagent A (green), 1x120ul Detection Reagent B (red), 1x120ul Assay Diluent A, 1x12ml Assay Diluent B, 1x12ml Substrate A, 1x10ml Positive Control, 1x1vial Substrate B, 1x2ml Wash Buffer (30X), 1x20ml

Store Microtiter plate, standard, detection reagents and postive control at -20°C. After reconstitution of standard control store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well. And then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 5. Aspirate and wash 5 times. 6. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C. 7. Read RLU value immediately.