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367174 IgG2a,k Mouse Isotype Control

Specifications
References
Purity
Purified by Protein A affinity chromatography.
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

367174 is a monoclonal immunoglobulin useful isotype control (negative control) for a comparative expression analysis including flow cytometry, immunoprecipitation, immunohistochemistry and immunocytochemistry in both human and mouse experimental models.

Applications
Suitable for use in a comparative expression analysis including Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry in both human and mouse experimental settings. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Application Information
Each lot of purified RPC5.4 has been routinely validated with four serially diluted concentrations to determine any non-specific binding in human PBMCs by flow cytometric analysis. It is suggested to use the same concentration of purified RPC5.4 isotype control immunoglobulin as that of IgG2a, k primary antibody.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
References
1. Ohno S, et al. 1977. J Natl Cancer Inst. 58:229. 2. Ohno S, et al. 1975. J Natl Cancer Inst. 55:569. 3. Percy ME, et al. 1976. Can J Biochem. 54:675.
USBio References
No references available
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