Suitable for use in a comparative expression analysis including Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry in both human and mouse experimental settings. Other applications not tested.

Optimal dilutions to be determined by the researcher.

Each lot of Biotin conjugated RPC5.4 has been routinely validated with four serially diluted concentrations to determine any non-specific binding in human PBMCs by flow cytometric analysis. It is suggested to use the same concentration of Biotin conjugated RPC5.4 isotype control immunoglobulin as that of IgG2a, k primary antibody.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.