When performing a PCR test, it is advantageous to confirm extraction of a valid biological template to allow accurate data analysis. The Precision qPCR endogenous control kit, contains a primer and probe mix designed to detect an endogenous gene in your species of interest. Detection of the endogenous control in your test sample confirms a high quality starting biological sample. Whereas a poor endogenous control signal may indicate that the sample did not contain sufficient biological material.
Sample Type
All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and DNA integrity. Always run at least one negative control with the samples. To prepare a negative-control, replace the template DNA sample with RNase/DNase free water.
Kit Components
Precision qPCR endogenous control primer/probe mix (150 reactions), 1x165ul RNase/DNase free water
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.