The Adalimumab (Humira®) BioAssay™ ELISA Kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices.

Serum and plasma

78-2500ng/ml

1.5ng/ml

250ng/ml of Adalimumab was spiked in 10 lots of human serum. Recovery ranges are from 90-109% with an average recovery of 104%.

Intra-assay CV: <10% Inter-assay CV: <10%

hIgG1, rituximab, and infliximab prepared at 250ng/ml were assayed and exhibited no crossreactivity or interference.

This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.

384854A: Microtiter Strips, 1x96 wells 384854B: Calibrator Diluent, 1x1.8ml 384854C: Calibrator (250ug/ml), 1x12ul 384854D: Wash Buffer, 10X, 1x50ml 384854E: Assay Buffer, 1x50ml 384854F: Detection Reagent, 1000X, 1x17ul 384854G: TMB, 1x12ml 384854H: Stop Solution, 1x12ml

Store kit components at -20°C unless specified otherwise. Unopened kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge small tubes on pulse setting prior to opening.