IGF-2 BioAssay™ ELISA Kit

Brand

BioAssay™

Kit Type

Sandwich ELISA

Tests

Sample Volume

50-100ul

Sensitivity

<2pg/ml

Detection Method

Colorimetric

Detection Range

62.5pg/ml-4000pg/ml

Sample Matrix

Cell culture supernates, serum, plasma (heparin or EDTA).

EU Commodity Code

38220000

Shipping Temp

Blue Ice

Storage Temp

4°C/-20°C

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. The rabbit IGF-II gene is located on chromosome 11. The standard product used in this kit is recombinant rabbit IGF-2, consisting of 67aa in mature form with the molecular mass of 7.5kD. IGF-1 and IGF-2 share high homology.

Sample Type

Cell culture supernates, serum, plasma (heparin or EDTA).

Intended Use

Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Rabbit IGF-2.

Sensitivity

<2pg/ml

Range

62.5pg/ml-4000pg/ml

Specificity

Recognizes natural and recombinant Rabbit IGF-2. There is no detectable cross-reactivity with IGF-1.

Test Principle

This Rabbit IGF-2 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit IGF-2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit IGF-2 amount of sample captured in plate.

Kit Components

96-well plate precoated with anti-Rabbit IGF-2 antibody, 1x plate Lyophilized recombinant Rabbit IGF-2 standard, 2x10ng/tube Biotinylated anti-Rabbit IGF-2 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer

Storage and Stability

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

References

1. Bachner-Melman, R.; Zohar, A. H.; Nemanov, L.; Heresco-Levy, U.; Gritsenko, I.; Ebstein, R. P. Association between the insulin-like growth factor 2 gene (IGF2) and scores on the Eating Attitudes Test in nonclinical subjects: a family-based study. Am. J. Psychiat. 162: 2256-2262, 2005. |2. Constancia, M.; Hemberger, M.; Hughes, J.; Dean, W.; Ferguson-Smith, A.; Fundele, R.; Stewart, F.; Kelsey, G.; Fowden, A.; Sibley, C.; Reik, W. Placental-specific IGF-II is a major modulator of placental and fetal growth. Nature 417: 945-948, 2002. |3. Ogawa, O.; Becroft, D. M.; Morison, I. M.; Eccles, M. R.; Skeen, J. E.; Mauger, D. C.; Reeve, A. E. Constitutional relaxation of insulin-like growth factor II gene imprinting associated with Wilms' tumour and gigantism. Nature Genet. 5: 408-412, 1993. |4. Dull, T. J.; Gray, A.; Hayflick, J. S.; Ullrich, A. Insulin-like growth factor II precursor gene organization in relation to insulin gene family. Nature 310: 777-781, 1984. |5. Brisken, C.; Ayyannan, A.; Nguyen, C.; Heineman, A.; Reinhardt, F.; Tan, J.; Dey, S. K.; Dotto, G. P.; Weinberg, R. A. IGF-2 is a mediator of prolactin-induced morphogenesis in the breast. Dev. Cell 3: 877-887, 2002. Note: Erratum: Dev. Cell 4: 283 only, 2003.

USBio References

No references available

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396700 IGF-2 BioAssay™ ELISA Kit (Insulin-like Growth Factor II) (Rabbit)

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