HOX4H
HoxD12 (homeobox D12), also known as HOX4H, is a 275aa protein that localizes to the nucleus and contains one homeobox DNA-binding domain. One of several members of the homeobox superfamily, HoxD12 functions as a sequence specific transcription factor that is important for the correct positioning of developing limb buds on the anterior-posterior axis.
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
Preparation before Usage
1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
Nucleotide Sequence
ATGTGTGAGCGCAGTCTCTACAGAGCGGGCTATGTGGGCTCGCTTCTGAATCTGCAGTCGCCAGACTCTTTCTACTTCTCCAACCTGAGGCCGAATGGCGGCCAGTTGGCCGCGCTTCCCCCTATCTCCTACCCGCGCGGCGCGCTGCCCTGGGCCGCCACGCCCGCCTCCTGCGCCCCCGCGCAGCCTGCGGGCGCCACTGCCTTCGGCGGCTTCTCGCAGCCCTACCTGGCTGGCTCCGGGCCTCTCGGCCTGCAGCCCCCAACAGCCAAAGACGGACCCGAAGAGCAGGCTAAGTTCTATGCGCCCGAAGCGGCCGCTGGGCCAGAGGAGCGCGGTCGTACCCGGCCGTCCTTCGCCCCCGAGTCTAGCCTGGCTCCTGCAGTGGCTGCTCTCAAAGCGGCCAAGTATGACTACGCTGGTGTGGGTCGTGCCACGCCGGGCTCCACGACCCTGCTCCAGGGGGCTCCCTGCGCCCCTGGCTTCAAGGACGACACCAAGGGCCCGCTCAACTTGAACATGACAGTGCAGGCGGCGGGCGTTGCCTCTTGCCTGCGACCTTCACTGCCCGACGGCCTGCCGTGGGGGGCGGCCCCGGGGAGGGCCCGCAAGAAGCGGAAACCCTACACGAAGCAGCAGATTGCGGAGTTGGAGAACGAATTCCTCGTCAACGAATTCATCAACAGGCAGAAACGCAAGGAATTGTCCAATAGGCTGAACCTCAGCGACCAGCAAGTCAAAATCTGGTTCCAGAACAGGCGTATGAAGAAGAAGCGCGTGGTGCTTCGGGAGCAGGCGCTGGCGCTCTACTAG
Translation Sequence
MCERSLYRAG YVGSLLNLQS PDSFYFSNLR PNGGQLAALP PISYPRGALP WAATPASCAP AQPAGATAFG GFSQPYLAGS GPLGLQPPTA KDGPEEQAKF YAPEAAAGPE ERGRTRPSFA PESSLAPAVA ALKAAKYDYA GVGRATPGST TLLQGAPCAP GFKDDTKGPL NLNMTVQAAG VASCLRPSLP DGLPWGAAPG RARKKRKPYT KQQIAELENE FLVNEFINRQ KRKELSNRLN LSDQQVKIWF QNRRMKKKRV VLREQALALY
Storage and Stability
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a lyophilized powder.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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